Characterization of ca/p layer produced on porous synthetic organic matrix by biomimetic method

摘要

A layer of Ca/P was produced on a porous cellulose sponge by making use of a biomimetic method. The first step, the exposure of cellulose to the bioactive glass (BAG) S53P4, was made at 37 deg C for 24 h with the substrate not in contact with the BAG. In the conventional method the substrate is contact with the BAg. Apatite growth was allowed to take place at the same temperature for varying periods of time up to 81 days. After 81 days treatment the apatite-layer covered most of the fibres of the organic matrix as indicated by modified von Kossa staining and by scanning electron microscopy (SEM), which revealed a homogenous but rough and hillocky layer of crystalline hydroxyapatite. The coating was further characterized by X-ray diffraction (XRD) and infrared spectroscopy (FTIR). XRD analysis indicated that formed Ca/P layer was amorphous and after 7 days crystallinity of the apatite-like layer was increased. FTIR showed that formed layer was carbonated hydroxyapatite.