In vivo bone ingrowth in rat marrow cell implanted macroporous calcium metaphosphate matrix

摘要

Bone formation was investigated by ectopic implantation of bioresorbable macroporous calcium phosphate (CMP) matrices combined with rat marrow cells of the athymic mouse. CMP matrices used were macroporous with about 200 mu m pore size. CMP matrices were soaked in the subcultured rat marrow cell suspension adjusted to 5 x 10~7 cells/ml for 30 minutes and then implanted into subcutaneous sites of an athymic mouse. The matrices recovered after 4 and 6 weeks and then uncalcified ground sections were prepared for histological examination. Osteogenesis could be observed in the pore regions at 4 weeks after implantation without fibrous encapsulation. This study thus suggested that the composite grafting of macroporous calcium metaphosphate matrices with marrow derived mesenchymal cells may be useful for repair massive bone defects.