Vectorial effects on selective cell adhesion of electrically poled hydroxyapatite ceramics

摘要

Biological behaviors, characterization and crystal growths on the electrically poled hydroxyapatite (HAp) ceramics were investigated. The specimens were electrically poled in a dc field. Bone-like crystal growths were studied in a simulated body fluid (SBF) with pH 7.25 at 36.5 deg C, and in an alpha minimum essential medium. Grown crystal layers were observed by scanning electron microscopy, and were analyzed by X-ray diffraction and Infrared spectroscopy. As a result, the bone-like HAp crystals grew rapidly on the negatively poled surface (N-surface), while the growth was restricted on the positively poled surface (P-surface) in SBF. It was considered that the aligned dipoles of substrate accelerated the crystal growth on the N-surface, and decelerated on the P-surface. Biological behavior was estimated by the cultivation of osteoblastic and fibroblastic cells on the surface of poled HAp. Cultured cells were found on the grown bone-like crystal layers for the N-surface, while the cells were directly on the specimens for the P-surface. On the poled HAp ceramics, N-surface had more adhered cultured cells than the P-surface.