Vectorial effects on tissue reaction of electrically poled hydroxyapatite ceramics

摘要

Poling effects of hydroxyapatite (HAp) ceramics were studied on histological reaction. The sintered HAp ceramics were cut and sliced for rectangular plates (5x8x0.5mm). These specimens were electrically poled (surface charged) in a DC field of 1000 V/cm for 1h. The poled HAp ceramics were implanted in bones of beagle dogs. The bones with the poled HAp samples were extracted after 3 and 7 days implantation. The histlogical reactions were estimated with the decalcified sections stained by hematoxylin eosin (H-E) and toluidine blue as well as the non decalcified sections by H-E, toluidine blue and Villanueva bone stain. New bone formations were observed on the negatively poled surface (N-surface). No contacted new bone formations but blood cell liners were observed on the positively poled surface (P-surface). Non poled HAp phases (0-surface) showed the intermediate reaction between the P- and N-faces. It was suggested that poled HAp vectored the cells and lured on the N-surface, whereas the P-surface had inhibitory effects on cell adhesion.