Visualization of laser-induced fluorescence of plants influenced by environmental stress with a microfluorescence imaging system and a fluorescence imaging lidar system

摘要

Abstract: Two different types of laser-induced fluorescence (LIF) imaging systems, a microfluorescence imaging system and a fluorescence imaging lidar system, have been developed for visualization of the fluorescence of plants to investigate and monitor their physiological status and so on. By using the microfluorescence imaging system, the distribution structures of the fluorescence inside tree leaves was obtained and the relationships among the cell/tissue locations, fluorescences and environmental stresses were investigated. Also, through outdoor experiments with the LIF imaging lidar, parameters related to the chlorophyll content of poplar leaves, which were about 60 m away from the system, were remotely estimated and visualized. It was shown that there was a close relationship between the results with both imaging systems and the LIF spectra emitted through leaf surfaces. The combination of the two systems will surely be a powerful tool for vegetation/plant monitoring. !3