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Near-field microscopy for medical a

机译:医用近场显微镜

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Abstract: An important approach dealing with sub lambda super resolution effect is based on a 3-D scanning of the examined sample using a pipette with small diameter. The scanning in the z direction is required for the conservation of the distance between the sample and the pipette and it is time consuming. In this paper we suggest a modification of that approach based on 2-D scanning. The scanning in the z direction is replaced by the usage of white light illumination. Based on the spectrum of the scanned information, one can compute the height of the surface. This consumes much less time than the conventional pipette scanner. This approach appears to be reasonable if the profile is varying not more than a portion of a wavelength. If, however, the object is not flat but slightly curved on a large scale we propose to employ a servo for the height. This servo can be much slower than the signal servo in the traditional approach. !20
机译:摘要:处理亚λ超分辨率效应的重要方法是使用小直径移液器对被检样品进行3D扫描。为了保持样品和移液管之间的距离,需要在z方向上进行扫描,这很耗时。在本文中,我们建议对基于二维扫描的方法进行修改。在z方向上的扫描被白光照明所替代。根据扫描信息的光谱,可以计算表面的高度。与传统的移液器扫描仪相比,这消耗的时间要少得多。如果轮廓变化不超过波长的一部分,则此方法似乎是合理的。但是,如果物体不是平坦的而是较大程度的弯曲,我们建议使用伺服器作为高度。该伺服可能比传统方法中的信号伺服慢得多。 !20

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