高山被孢霉突变株产花生四烯酸培养条件的研究

摘要

With temperature, pH, shaking speed and culture time as four influencing factors, and bio-mass and productivities of oil and arachidonic acid ( ARA) as comprehensive evaluation indexes, the cul-ture conditions of Mortierella alpina mutant 12-2-2 to produce ARA were studied by single factor ex-periment and orthogonal experiment. The results showed that temperature had the greatest influence on the ARA production by Mortierella alpina mutant 12-2-2, followed by culture time, shaking speed and pH;the optimal culture conditions were obtained as follows: temperature 26℃, culture time 7 d, sha-king speed 160 r/min and pH 8. Under these conditions,the biomass and productivities of oil and ARA were 32. 31 g/L, 12. 98 g/L and 5. 12 g/L, respectively.%为了研究高山被孢霉突变株12-2-2产花生四烯酸( ARA)的培养条件,选取温度、pH、摇床转速和培养时间为4个影响因素,以生物量、油脂产量和ARA产量为综合评价指标,进行单因素实验和正交实验。结果表明,影响高山被孢霉突变株12-2-2产ARA的因素主次顺序为：温度﹥培养时间﹥摇床转速﹥pH；最优培养条件为：温度26℃,培养时间7 d,摇床转速160 r/min,pH 8。在最优培养条件下,高山被孢霉突变株12-2-2的生物量为32.31 g/L,油脂产量为12.98 g/L,ARA产量为5.12 g/L。