基于Label-free技术的鹿角与鹿骨蛋白组分比较

摘要

旨在利用Label-free技术对天山马鹿的鹿角与鹿骨蛋白组分进行比较分析.以脱盘后130 d的马鹿鹿角和鹿骨为研究对象,利用Label-free蛋白质组学技术和生物信息学方法解析比较鹿角与鹿骨的蛋白质组分.结果共成功鉴定1 138种蛋白质,其中鹿骨蛋白934种、鹿角蛋白835种.通过对成功鉴定到的蛋白质进行功能注释发现,鹿骨蛋白特异富集到了低密度脂蛋白受体代谢、黑质体发育、血管再生、细胞凋亡信号通路调节等过程;而鹿角特有蛋白富集到了肽类激素加工过程、活化T细胞增殖调节等生物学过程,其中肽类激素加工过程主要蛋白有CPE、CPN1、ECE1,活化T细胞增殖调节蛋白主要有IGF2、PRKAR1A、PYCARD.利用SPSS软件筛选鹿角与鹿骨显著差异表达蛋白(差异倍数＞2,P＜0.05),共筛选出差异蛋白质335种,其中75种蛋白在鹿骨中表达上调,260种蛋白在鹿角中表达上调,其中cathelicidin 1与cathelicidin 5在鹿角中为高表达,而cathelicidin 6在鹿骨中高表达.该试验结果弥补了鹿角与鹿骨蛋白质组分研究的空白,为进一步研究鹿角与鹿骨的有效成分奠定基础.%The study aimed to carry out the comparison proteomic analysis of wapiti antler and bone based on Label-free.In this paper,the wapiti antlers and bone aged 130 d were selected as experimental material,then the protein components of antler and bone were analyzed by using the Label-free proteomics methods and bioinformatics methods.The results showed that a total of 1 138 proteins were identified,including 934 deer bone proteins and 835 antler proteins.Through the functional annotation of proteins successfully identified,deer bone proteins were specifically enriched in the process of low-density lipoprotein receptor particle metabolism,substantia nigra development,angiogenesis and regulation of cell apoptotic signaling pathway.The antler proteins were enriched in the peptide hormone processing,regulation of activated T cell proliferation and other biological processes.The proteins related to peptide hormone processing process were CPE,CPN1,ECE1,and the proteins related to regulation of activated T cell proliferation were IGF2,PRKAR1A,PYCARD.A total of 335 differentially expressed proteins (fold change＞2,P＜0.05) were screened out by SPSS software.Among them,75 proteins were up-regulated in deer bone and 260 proteins were up-regulated in antlers.In addition,cathelicidin 1 and cathelicidin 5 were highly expressed in antlers,while cathelicidin 6 was highly expressed in the deer bone.The results fill the blank in the study of antler and deer bone protein components,and lay the foundation for the further study on the pharmacological active substances of antler and deer bones.