利用白色杜洛克×二花脸资源家系定位影响猪肉硬实度的QTL

摘要

为定位影响猪背最长肌和半膜肌硬实度的数量性状基因座(QTL),本研究测定了白色杜洛克×二花脸资源家系中490头F_2代猪的背最长肌和半膜肌硬实度,检测了3代个体在19条染色体上194个微卫星的基因型,并据此进行了QTL定位分析.共检测到9个QTLs,包括影响背最长肌硬实度的4个QTLs,分别位于SSC1、SSC2、SSC7和SSC10,其中SSC1和SSC7的QTLs达到1%基因组显著水平;影响半膜肌硬实度的5个QTLs分别位于SSC1、SSC7、SSC10、SSC12和SSC14,其中SSC7和SSC14的QTLs分别达到5%和1%基因组显著水平.%To identify quantitative trait loci(QTLs)for firmness of M. Longissimus dorsi (LD) and M. Semimembranous (SM) in pigs, the related traits were measured in 490 F_2 animals from a White Duroc×Erhualian resource population. A whole genome scan was performed with 194 microsatellites on 19 porcine chromosomes, and the QTL analysis was performed based on a least-square regression method. A total of 9 QTLs were identified,including 4 QTLs for firmness of LD on SSC1, SSC2, SSC7 and SSC10, those on SSC1 and SSC7 reached the 1% genome-wide significant level. Five QTLs for firmness of SM were detected on SSC1, SSC7, SSC10, SSC12 and SSC14, the QTLs on SSC7 and SSC14 showed 5% and 1% genome-wide significant effects respectively.