内源性绵羊肺腺瘤病毒基因在蒙古羊染色体上的分布研究

摘要

This study was aimed to explore the distribution of chromosomes of the endogenous sheep pulmonary adenomatosis virus gene in Mongolian sheep. A pair of primers was designed ac-cording to the sequence of the enJSRV in the GenBank(DQ838493). The partial sequence of the gag gene was cloned and amplified by PCR, which was further marked by Dig to locate the distri-bution of the enJSRV in the Mongolian sheep chromosomes with the FISH (Fluorescence in situ hybridization). The result showed that the distribution of the enJSRV gag gene in each of the 6 chromosomes of Mongolian sheep during the metakinesis at 1q45, 1p23, 2q41, 3q23, 6q13, 9q22 and 14q25, and stronger fluorescence signal appeared on the 6th and 9th chromosomes. The re-suits suggested that, on the 6th and 9th chromosomes, enJSRV genes were multiple copied at the two sites, and there were low copy number at 1q45, 1p23, 2q41, 3q23, and 14q25, which related to the endogenous evolution of the sheep pulmonary adenomatosis virus.%本研究旨在探究内源性绵羊肺腺瘤病毒基因在蒙古羊染色体上的分布情况.参照已登入GenBank中的内源性绵羊肺腺瘤病毒(enJSRV,登录号为DQ838493)序列,设计合成1对引物,PCR扩增gag基因的部分片段,用地高辛标记制备探针,利用荧光原位杂交(FISH)技术检测enJSRV在蒙古羊染色体上的分布.结果表明,用en-JSRV的gag基因合成的DNA探针在蒙古羊中期分裂相的6条染色体上均有杂交信号,分别为1q45、1p23、2q41、3q23、6q13、9q22和14q25,其中在6和9号染色体上出现有较强的荧光信号.结果提示,在6和9号染色体上这2个位点上有多拷贝的enJSRV基因,1q45、1p23、2q41、3q23、和14q25拷贝数少,这与内源性绵羊肺腺瘤病毒进化有关.