甘肃合作猪白细胞抗原经典Ⅱ类分子DR基因克隆及生物信息学分析

摘要

Class Ⅱ DRA and DRB genes of swine leukocyte antigen (SLA) of Gansu Hezuo pigs were cloned, and their characteristics and polymorphism were analyzed to explore immunological basic parameters for xenotransplantation from pigs to humans. SLA-DRA and SLA-DRB genes of Hezuo pigs were cloned by RT-PCR, sequenced and submited to Genbank (accession numbers: FJ905823 and FJ9058258). Analysis results through BLAST in NCBI and related software in Ex-PASy suggested that this kind of inbred strain of pig would be the animal model of choice for the porcine-human xenotransplantation after modification with gene engineering methods.%为了克隆甘肃合作猪白细胞抗原(SLA)DRA和DRB基因,分析SLA-DR基因特性和抗原多态性,首次研究了甘肃合作猪在异种移植等研究领域中的应用前景.应用RT-PCR分别扩增合作猪SLA-DRA和SLA-DRB基因并进行测序,将所获得基因序列登录GenBank(登录号分别为FJ905823和FJ9058258),再用NCBI中的BLAST和ExPASy等相关软件进行生物信息学分析.发现在猪-人异种移植中,近亲繁殖的合作猪种在与人类主要组织相容性复合体遗传基因水平相似性方面有一定优势,也可作为备选猪种对相关基因进行必要的修饰和改造.