IL-15单核苷酸多态性与儿童急性淋巴细胞白血病治疗反应相关性

摘要

目的探讨白介素15（IL-15）单核苷酸多态性（SNP）位点与中国儿童急性淋巴细胞白血病（ALL）治疗反应的相关性。方法提取ALL患儿缓解期的骨髓细胞基因组DNA，采用质谱阵列技术（MassArray）分析IL-15的5个SNP位点（rs10519612、rs10519613、rs17007695、rs17015014、rs35964658），并对各SNP位点与诱导治疗结束后微小残留病（MRD）进行相关性分析。结果SNP rs17007695与儿童ALL早期诱导治疗结束后的MRD相关（P=0.049），CC基因型诱导结束时MRD阳性率是TT基因型的1.8倍；5个SNPs的单倍型分析显示，CACGG在MRD阳性组中出现的频率高于MRD阴性组，差异有统计学意义（10.5%对4.9%，P=0.035）。结论IL-15相关的SNP与儿童ALL早期治疗反应相关。%Objectives To investigate the correlation between single nucleotide polymorphisms (SNP) in interleu-kin-15 (IL-15) and treatment response in childhood acute lymphoblastic leukemia (ALL). Methods Genomic DNA samples extracted from remission bone marrow cells of ALL patients were genotyped by MassArray. Five SNPs (rs10519612, rs10519613, rs17007695, rs17015014 and rs35964658) in IL-15 and their association to minimal residual disease (MRD) status in the end of induction therapy were studied. Results SNP rs17007695 was associated with the early response in children with ALL(P=0.049) and the incidence of positive MRD after induction therapy in CC genotype carriers was 1.8 times more than that in TT genotype carriers. Haplotype analysis of these five SNPs showed that the frequency of haplotype CACGG in MRD positive group was 2.1 times higher than that in MRD negative group (P=0.035). Conclusions IL-15 gene polymorphism was associated with the early treatment response in Han Chinese children with acute lymphoblastic leuke-mia.