目的观察母代高脂饮食和后天高脂饮食对子代大鼠代谢状态的不同影响，以探索母代高脂饮食与子代大鼠成年后代谢性疾病发生的关系。方法将成年SD大鼠分成对照组（C组）和高脂饮食组（H组），至10周龄时交配怀孕生育子代大鼠，子代大鼠断乳后每组再分成高脂饮食组（HH组，CH组）和普通饮食组（HC组，CC组），分别至子代大鼠3周龄和8周龄时观察其代谢相关性指标以及肝脏病理表现。结果H组母鼠孕前体质量、糖耐量曲线下面积、总胆固醇及三酰甘油均明显高于C组母鼠（P<0.05）；3周龄时H组母鼠所生育并喂养的子代大鼠其体质量明显高于C组母鼠生育的子代大鼠（P=0.002），但两者的糖耐量曲线下面积差异无统计学意义（P＞0.05）；至8周龄时各组子代大鼠空腹血糖和胰岛素水平差异无统计学意义，而母鼠为H组的子代大鼠体质量和糖耐量曲线下面积大于母鼠为C组子代大鼠（母代饮食主效应P分别为0.024和0.013），HH组和CH组子代大鼠糖耐量曲线下面积亦大于HC组和CC组的子代大鼠（子代饮食主效应P=0.041）；8周龄时HH组和CH组子代大鼠血清总胆固醇、三酰甘油和低密度脂蛋白水平均高于HC组和CC组（子代饮食主效应P分别为0.008，0.007和0.000）；8周龄时HH组、HC组和CH组子代大鼠表现出不同程度的脂肪肝；CC组子代大鼠肝脏镜下结构均显示正常。结论母代高脂饮食可以引起子代大鼠成年后体质量增加，糖耐量减退以及肝脏脂肪变，增加子代发生代谢性疾病的危险性。%Objectives To examine the association of the maternal high-fat (HF) diet with increased susceptibility to obe-sity and the development of metabolic diseases in their offspring, and observe difference in the effect of maternal vs. acquired high fat diet on metabolic state in their offspring. Methods A total of 15 SD female rats were divided into HF diet group (group H, n=9) and control diet group (group C, n=6). After fed on different diet for seven weeks, they were mated at the age of ten weeks and became pregnant. Their offspring were then divided to groups CH and HH fed HF diet and groups CC and HC fed control diet. At the age of 3 and 8 weeks, the metabolic markers and the liver pathohistological evidences of their offspring were obtained. Results The body weight, area under curve (AUC) of glucose tolerance, cholesterol and triglyceride were all higher in group H than those in group C (P<0.05) before pregnancy. The offspring of group H had a higher body weight than the offspring of group C at the age of 3 weeks (P=0.002), and no difference in AUC was found between two groups (P>0.05). At the age of 8 weeks, there was no difference in fasting glucose and insulin levels among the four offspring groups. The AUC and body weight were higher in group H than in group C (main effect of maternal diet, P=0.024, P=0.013). The AUCs were also higher in groups CH and HH than groups CC and HC respectively (main effect of acquired diet, P=0.041). The levels of total cholesterol, triglycerides and LDL at the age of 8 weeks were all higher in HH and CH groups than those in HC and CC groups (main effect of acquired diet, P=0.008, 0.007, 0.000, respectively). Their histological analysis at 8 weeks showed different degrees of fatty liver in HH, HC and CH groups, and normal liver in CC group. Conclusions Maternal HF diet may result in increased body weight, fatty liver and impaired glucose tolerance in their adult offspring, and thus increase the risk of developing metabolic diseases at their later age. .
展开▼
