Objective To investigate the role of Astragahs in alleviating adhesion of neutrophils to HK-2 cells induced by postasphyxial-serum of neonate and its signal transduction mechanism. Methods HK-2 cells were used as target cell. Control group, asphyxia group, Astragalus group were divided in the experiment. The 20% (volume fraction) postasphyxial-serum was used as attacking factor. The following indicators were detected: cellular morphology and neutrophils adhesion were observed with inverted microscope. The activity of myeloperoxidase (MPO) in the cell suspension were determined by biochemistry assay as the indicator for adhesion of neutrophils to HK-2. Intercellular adhesion molecule-1(ICAM-1) were examined by flow eytometer. Results The neutrophils numbers (MPO activity) and ICAM-1 expression in asphyxia group were higher than that of the control group, but dramatically decreased in Astragalus group (P < 0.05). Conclusions These data demonstrated that Astragalus could alleviate the adhesion of neutrophils from neonate with asphyxia to HK-2 and it' s intracellular signal transduction mechanism is presumably involved in the inhibition of ICAM-1 expression on HK-2 cellular membrane.%目的 研究黄芪减轻新生儿窒息后血清诱导的HK-2细胞黏附中性粒细胞的作用及信号转导机制.方法 以HK-2细胞为研究对象,实验细胞分为对照、窒息和黄芪干预组3组,用含20%(体积比)窒息后血清作为攻击因素.采用倒置相差显微镜下观察HK-2细胞损伤及黏附中性粒细胞的情况,用生化法检测细胞悬液中髓过氧化物酶(MPO)活性;采用流式细胞术检测细胞间黏附因子-1(ICAM-1)的表达情况.结果 与对照组比较,窒息组HK-2细胞黏附较多中性粒细胞(MPO活性增加),ICAM-1表达增加,差异有统计学意义(P均<0.05);与窒息组比较,黄芪干预组HK-2细胞黏附中性粒细胞较少(MPO活性下降),ICAM-1表达减少,差异具有统计学意义(P均<0.05).结论 黄苠具有减轻窒息后血清诱导HK-2细胞黏附中性粒细胞的作用,其机制可能与抑制HK-2细胞表面ICAM-1表达有关.
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