A 1400bp DNA fragment in 5’ region of Toc33 Brassica napus was cloned by an improved single primer PCR method.The result of DNA sequence analysis showed that the fragment consisted of two regions.One of 491bp was partial coding sequence of Toc33 gene,the other of 909bp was the promoter of Toc33 gene.Besides TATA-box and CAAT-box,the promoter sequence included several cis-acting elements which had relation to light-regulation of plant.The cis-acting elements were G-box,GATA-box,I-box,SORLIP1AT motif,CIACADIANLELHC motif and so on.As a result,it was presumed that the transcription activity of promoter of Toc33 gene from Brassica napus may be regulated by light.
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