Objective The purpose of the study was to observe the effect of glycyrrhiza. on migrating myoelectric complex (MMC) in small intestine in rats and to investigate the mechanism through which the effect oecours. Methods Twenty adult SD rats were randomly divided into bethanoechol group and glycyrrhiza group. MMC in small intestine in rats was measured be fore and after drug administration. Another 20 rats were randomly divided into normal control group and glycyrrhiza group. The content of 5-hydroxyptamine(5-HT) in enteroehromaffin cell(EC) and enteric nervous system(ENS) was measured by immunohistoehemical method in the two groups and the immunoreactivity in EC and ENS was determined quantatatively by microspectrophotometry. Results G lycyrrhiza inhibited and guarded against the contration of duodenum and jejunum induced by bethanechol. The content of 5-HT in EC and ENS in glycyrrhiza group was significantly lower than that of normal control group ( P < 0.05) .The inhibition of MMC in small intestine in the rat was caused by the glycyrrhiza which inhibits the 5-HT secretion by phenoehromoeytes and the level of 5-HT in ENS. Conclusion One of the mechanisms of glycyrrhiza inhibiting MMC in duodenum and jejunum in the rats may be involved in postsynaptic inhibition induced by M-receptor. The another mechanism of glycyrrhiza inhibition MMC in small intestine was that the secretion of 5-HT in EC and the content of 5-HT in ENS were decrcased by glycyrrhiza. Thus the inhibition of the MMC in small intestine occurs.%目的：观察甘草对大鼠小肠移行性综合肌电(MMC)的影响，并探讨其作用机制。方法：20只SD大鼠平分为氨甲酰甲胆碱组和甘草组，用药前后检测小肠的电活动，以MMC活动周期、Ⅲ相持续时间为指标分析小肠电活动状态。另20只SD大鼠平分为正常组和甘草组，用免疫组织化学法检测小肠嗜铬细胞及小肠神经系统(ENS)中5-HT的含量，用显微分光光度计对免疫染色强度进行定量测定。结果：(1)甘草能抑制肌受体激动剂氨甲酰甲胆碱引起的十二指肠和空肠收缩反应，并能预防其收缩反应的发生；(2)甘草组与正常组比较，小肠嗜铬细胞和ENS中5-HT含量明显减少。结论：(1)甘草对大鼠十二指肠和空肠的抑制作用机制之一可能是由M-受体介导的突触后抑制作用；(2)甘草通过抑制小肠嗜铬细胞分泌5-HT及降低ENS中5-HT的含量而对大鼠小肠MMC活动起抑制作用
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