Objective To establish the reference intervals of serum 25-hydroxyvitamin D [25(OH)D] among minors in Shanghai. Methods A total of 400 minors (≤16 years old) were enrolled,including 200 boys and 200 girls. They were classified into 5 groups according to ages,<1 month(20 cases),1-<6 months(80 cases),6 months-<3 years (100 cases),3-<6 years(100 cases)and 6-16 years(100 cases). The level of 25(OH)D was determined by enzyme-linked immunosorbent assay (ELISA). Results In the results of 400 cases,1 outlier was eliminated. The 25(OH)D levels demonstrated skewed distribution,so its overall level was shown by median (M)[quartiles(P25-P75)]. The 25(OH)D level of 399 cases was 55.64(42.93-76.05) nmol/L,56.47(44.59-76.48) nmol/L for boys and 55.44(41.07-75.55) nmol/L for girls. There was no statistical significance between boys and girls(P>0.05). There was no statistical significance for different ages (P>0.05). The 25(OH)D level in the groups of 1-<6 months and 6 months-<3 years were higher than those in the other 3 groups(P<0.05). The reference intervals should be established according to ages. The reference intervals were shown by percentiles [the 2.5th percentile(P2.5)-the 97.5th percentile (P97.5)] and were 32.66-79.33 nmol/L for <1 month group,32.45-129.91 nmol/L for 1-<6 months group,38.98-129.90 nmol/L for 6 months-<3 years group,32.22-86.44 nmol/L for 3-<6 years group and 30.74-87.74 nmol/L for 6-16 years group. Conclusions The reference intervals of 25(OH)D have been initially established for minors in Shanghai.%目的 初步建立上海地区未成年人血清25-羟基维生素D[25(OH)D]的参考区间.方法 选取≤16岁的未成年人400名,男、女各200名.按年龄分为<1个月(20名)、1~<6个月(80名)、6个月~<3岁(100名)、3~<6岁(100名)、6~16岁(100名)5组.采用酶联免疫吸附试验(ELISA)检测血清25(OH)D.结果 400名未成年人25(OH)D结果中剔除1个离群点,总体呈偏态分布,因此以中位数(M)[四分位数(P25~P75)]表示.399名未成年人血清25(OH)D水平为55.64(42.93~76.05)nmol/L,男、女童分别为56.47(44.59~76.48)和55.44(41.07~75.55)nmol/L,二者之间差异无统计学意义(P>0.05),且各年龄组内男、女童之间差异亦无统计学意义(P>0.05).因此,计算参考区间时可忽略性别差异.1~<6个月组和6月~<3岁组25(OH)D水平明显高于其他3组(P<0.05).因此,需按年龄分别建立参考区间.以百分位数法[第2.5百分位数(P2.5)~第97.5百分位数(P97.5)]建立各年龄组的参考区间:<1个月为32.66~79.33 nmol/L,1~<6个月为32.45~129.91 nmol/L,6个月~<3岁为38.98~129.90 nmol/L,3~<6岁为32.22~86.44 nmol/L,6~16岁为30.74~87.74 nmol/L.结论 初步建立了本地区未成年人25(OH)D的参考区间.
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