呼吸道病毒实验室检测技术的研究进展

摘要

呼吸道病毒是威胁人类健康的主要因素之一，因此快速准确地鉴别病原微生物具有重要的临床意义。文章介绍了目前应用于呼吸道病毒检测的一些经典或新兴实验室技术，包括病毒分离培养技术、免疫学技术、核酸扩增技术、芯片技术、下一代测序技术、质谱（ MS）技术等。这些技术以其各自的特点应用于呼吸道病毒的临床诊断、疗效观察以及科学研究与探索。%Respiratory virus is one of main factors that threatens human health, so it is significant to identify pathogenic microorganisms accurately and rapidly.This article introduces some traditional or new laboratory testing technologies that have been applied to respiratory virus determination nowdays, including virus isolation culture technique, immunological technique, nucleic acid sequence amplification technique, chip technology, next generation sequencing technique, mass spectrometry ( MS ) technique and so on.These technologies are applied into clinical diagnosis, curative effect observation, scientific research and investigation of respiratory virus by their characteristics.