385例癌患者自身抗体检测结果分析

摘要

Objective To study the positive rates of autoantibodies in thepatients with malignancies as well as the locations of their target antigens in cells and identify the essence of their target antigens. Methods Sera of 385 malignancy patients as well as 140 healthy controls were detected with indirect immunofluorescence (IIF) method using antigens extracted from human epithelial cells (Hep-2). Results We detected 95 patients' sera with positive IIF results with westernblotting method. There are significant differences in positive rates, fluorescent patterns, and location of autoantigens in cells between concer group and health group(P＜0.05). Although with age growing, both have increasingly higher positive rates, concer group are much higher than those of control in every age group(P＜0.01). There is no significant difference among different malignancy groups(P＞0.05). Among 95 patients' sera with positive IIF results,90 of them were objected one or more positive bands. A few bands were confirmed as several known autoantibodies usually appearing in some autoimmune diseases according to standards provided by the kit, which included PM-SCL(2), Ku(7), CENP(5), M2(9)， RNP(4)， SS-A(7)， Jo-1(1), SS-A/SS-B(5), Rib-P-Protein(4). The autoantigen of most other bands were still unknown. Conclusion Dispite of age-related, autoantibodies in malignancy patients are malignancy-related. They are different from those in healthy people as well as patients with autoimmune disease. Further efforts should be made to identify the target antigens as well as their biological role and clinical significance.%目的 了解癌患者体内自身抗体阳性率、细胞内定位及荧光图形特点，初步确定自身抗体靶抗原本质，了解自身抗体与癌的相关性。 方法 应用间接免疫荧光法(IIF)检测385例9种癌的患者以及140份不同年龄段正常人血清中自身抗体；以人喉癌上皮细胞(Hep-2)全细胞裂解液为抗原，应用免疫印迹法，对95份经IIF检测为自身抗体阳性的癌患者血清进行分析。 结果 癌组与对照组在阳性率、荧光图形、靶抗原细胞内定位上均有显著差别，两组人群的自身抗体阳性率皆随年龄增长而增高(P＜0.05)，而不同年龄段的癌患者组皆显著高于对照组(P＜0.01)；不同癌种间，上述结果差异皆无显著性(P＞0.05)。95份IIF阳性血清中，90份出现1条或多条阳性条带，其中多发性肌炎硬皮病自身抗体(抗PM-SCL)2份、抗Ku7份、抗着丝点蛋白抗体(抗CENP5份)、线粒体抗体2型(抗M2)9份、核糖蛋白抗体(抗RNP)4份、干燥综合征A抗原抗体(抗SS-A)7份、组氨酰-tRNA合成酶抗体(抗Jo-1)1份、抗SS-A/SS-B5份、核糖体P蛋白抗体(抗Rib-P-Protein)4份，而另有多数条带对应靶抗原未明。 结论 提示癌患者体内自身抗体具年龄相关性和癌种相关性。其自身抗体谱不同于正常人生理性自身抗体谱及自身免疫性疾病患者体内自身抗体谱。进一步研究自身抗体与癌的相关性，明确未知靶抗原本质，有助于深入认识癌患者的自身免疫现象。