目的 探讨一种快速筛选菌尿的方法。 方法 用10μl尿液直接涂片，经革兰染色后镜检，同时取10μl尿液做细菌培养及菌落计数。 结果 总计检测1155份尿标本，其中显微镜检查阳性(每个油镜视野平均菌数≥2)且尿培养阳性(菌落计数≥105cfu/ml)的标本有100份，两者均阴性的有1047份；6份涂片阳性而培养阴性；2份涂片阴性而培养阳性，显示涂片镜检法灵敏度98.0%，特异性99.4%，阳性预示值94.3%，阴性预示值99.8%。 结论 10μl尿液直接涂片临床检查菌尿症是一种快速、有效、简便的方法。%Objective To develop a rapid method for the detection ofbacteriuria. Methods 10 μl of urine sample was smeared on the slide, examined by microscopy after Gram stain; 10 ul of urine sample was examined by cul-ture. Results 100 samples showed positive in both microscopic examination (≥2 bacteria per oil immersion field) and cultures (colony counts of ≥105 CFU/ml); 1047 samples both negative; six samples microscopic examination positive and cultures negative; two samples microscopic examination negative and cultures positive. So the resolved sensitivity and specificity of the microscopic examination are 98.0% and 99.4% respectively; Positive predictive value and negative predictive value are 94.3% and 99.8% respectively. Conclusion The method for detecting significant bacteriuria by microscopic examination with 10 μl sample is rapid, effective and simple.
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