胃肠道肿瘤患者血浆组织因子的含量和mRNA的表达

摘要

目的探讨血浆中组织因子(Tissue factor,TF)的检测对胃肠道肿瘤患者的临床意义。方法采用ELISA法测定32例胃癌、45例结直肠癌患者和30例正常人血浆中TF，并应用逆转录-聚合酶链反应(PT-PCR)检测这些患者和正常人外周血单核细胞中的mRNA。结果两组消化道肿瘤患者血浆中的TF均远远高于正常组，与病理分级并无相关性；采用RT-PCR法在大部分肿瘤患者单核细胞中可检测到TFmRNA，但在正常组中均不能检测到。结论肿瘤患者血浆中TF升高可能是由于单核细胞合成增加所致，检测TF在血浆中的水平和其mRNA的表达将有助于研究肿瘤发生发展的机制。%Objective　 To investigate the serum level of tissue factor (TF) and mRNA expression in monocyte from patients with gastrointestinal tumor. Methods The TF level was determined in 32 gastric cancers, 45 colorectal carcinomas, 30 normal persons were determinated with ELISA, RT-PCR was used to detect expression of mRNA in monocyte. Results The high serum TF level was found in gastrointestinal tumor. TF mRNA in monocyte can be detected in most of cancer patients whereas it is undetectable in normals. Conclusions Expression of TF mRNA in monocyte probably accounts for high level of TF level in serum, TF value and mRNA expression can be a useful tool for early diagnosis and studying pathological processes.