免疫印迹法检测血清抗Sa抗体方法的建立

摘要

Objective To establish the method to examine the anti-Sa antibody and study its value in diagnosing rheumatoid arthritis (RA). Method The optimal conditions to examine the anti-Sa antibody by Western blot have been selected through three groups of complete cross tests. We examined the anti-Sa antibody in the sera of patients with RA or other autoimmunity diseases and healthy people. The RF was examined by immuno latex agglutination. Results The sensitivity of anti-Sa antibody to RA is 42.7% and its specificity is 100%, the sensitivity of RF is 72.0% and its specificity is 79.2%. Conclusion The specificity of the anti-Sa antibody is higher than that of RF, so the diagnostic value of anti-Sa antibody was better than that of RF.%目的建立抗Sa抗体的检测方法，研究抗Sa抗体对类风湿性关节炎(RA)的诊断意义。方法通过3组正交试验选择免疫印迹法检测抗Sa抗体的最适条件，并用该法检测RA、其他自身免疫病及健康人血清，用免疫胶乳凝集法检测血清中的RF。结果抗Sa抗体对RA的敏感性为42.7%，特异性为100%；RF对RA的敏感性为72.0%，特异性为79.2%。结论抗Sa抗体对RA的特异性高于RF与RF结合使用有利于RA的明确诊断。