Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes;

摘要

There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, andcompletely based on biologically-derived materials could offer such potential. Here we report a simpleand modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficientlycombines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines;