Development of Newer Methods for the Isolation and Identification of Certain Components Found in Complex Mixtures Derived from Energy Sources and the Determination of Their Biological Activity Via Bioassay Systems. Final Summary,

摘要

Multidimensional capillary column gas chromatography proved to be capable of readily separating more individual components of complex mixtures than any other system. By utilizing the technique of Sample Trapping and/or Sample Transfer, a single component or a series of components can be held in obeyence and at will, be quantitatively transferred to either/or (1) another column (2) another detector (3) a mass spectrometer (or any other appropriate identification device) and (4) finally and most importantly, to a suitable bioassay system. With Sample Trapping, the segment of sample of interest is first trapped out in a small volume loop of inert tubing at liquid CO sub 2 temperatures and at an appropriate time it is rapidly heated. The volatilized is directed to the inlet of column No. 2. Under these circumstances, oft times band broadening is minimized and superior resolution of all of the component bands is achieved on the second column. For Sample Enrichment, repeated injections of the same are automatically cut for components of interest until sufficient sample is present in the trap for analysis via High Resolution Mass Spectrometry or NMR. (ERA citation 09:005079)