外周血片涂抹细胞的实验室解决思路

摘要

目的 探讨减少外周血片涂抹细胞产生的实验室解决思路.方法 收集20例慢性淋巴细胞白血病(CLL)患者的乙二胺四乙酸二钾抗凝血,使用血液分析仪及自动推染片机根据不同角度及速度自动上样制作外周血片,通过DM96全自动血细胞形态学分析仪进行阅片,计数涂抹细胞数量;在添加不同比例的胎牛血清处理后,比较涂抹细胞的数量变化.结果 采用27°角、速度为140 m/s时制片涂抹细胞最理想.胎牛血清与外周血以1:4的比例处理后,涂抹细胞明显减少.结论 当外周血片出现大量涂抹细胞时,可通过选择合适的角度、速度及联用添加胎牛血清的方法来减少涂抹细胞的产生,从而为临床提供更为准确的分类计数结果.%Objective To investigate the solutions for decreasing the number of smudge cells in peripheral blood smears.Methods Samples was collected in ethylene diamine tetraacetic acid-K2 anticoagulation tubes from 20 patients with chronic lymphocytic leukemia(CLL). Blood smears were automatically prepared with each angle and speed by hematology analyzer and automated slide maker and stainer. Smudge cells were counted by DM96 automated blood cell morphometry analyzer. After treatment with different ratios of fetal bovine serum,the results were compared.Results It is better to decrease the number of smudge cells with the speed of 140 m/s at 27 degrees. When the ratio of fetal bovine serum to peripheral blood was 1:4,the number of smudge cells was decreased. Conclusions When a large number of smudge cells are appearing in peripheral blood smears,it should not only use an appropriate angle and speed,but also add fetal bovine serum to decrease the number of smudge cells,so as to provide an accurate classification and counting results for clinical practice.