摘要:目的:探讨白细胞介素-32(Interleukin ,IL-32)在大鼠肾缺血再灌注(ischemia-reperfusion injury ,IRI)损伤中的作用及其机制。方法:80只雄性SD大鼠随机分为4组:假手术组(S组,n=20)、缺血再灌注组(I/R组,n=20)、缺血前 IgG 抗体预处理组(IgG+I/R组,n=20)和缺血前IL-32抗体预处理组(Anti-IL-32+I/R组,n=20)。采用夹闭双侧肾蒂30 min 后恢复血供的方法制备肾脏缺血再灌注损伤模型,再灌注3h、6h、12h、24h分别处死大鼠收集血样和肾脏样本,自动生化分析仪测定血清中肌酐(Cr)、尿素氮(BUN)浓度,酶联免疫吸附试验(ELISA)检测大鼠血清IL-32、TNF-α、IL-1β的表达,化学比色法检测肾组织髓过氧化物酶(MPO)的活性。结果:与S组比较:1、血清Cr、BUN 浓度,I/R、IgG+ I/R组均显著升高(P<00.5),Anti-IL-32+ I/R组无显著性差异(P>00.5);2、血清IL-32、TNF-α、IL-1β水平,I/R、IgG+ I/R组均显著升高(P<00.5),Anti-IL-32+ I/R组无显著性差异(P>00.5);3、肾组织 MPO 活性,I/R、IgG+ I/R组显著增强(P<00.5),Anti-IL-32+ I/R组无显著性差异(P>00.5)。结论:IL-32在肾脏缺血再灌注损伤后的大鼠血清中高表达,阻断IL-32能减少炎症因子释放、抑制中性粒细胞聚集,减轻肾功能损害。%Objective:To investigate the effect of IL-32 on renal ischemia-reperfusion injury and its mech-anism in rats .Methods :Eighty male SD rats were randomly divided into 4 groups :Sham group(S group ,n=20) ,renal IRI group(I/R group ,n=20) ,IgG preconditioning before ischemia group (IgG+I/R group ,n=20) and Anti-IL-32 preconditioning before ischemia group (Anti-IL-32+I/R group ,n=20) .Both renal pedicales of rats were clamped for 30 minutes to make the animal models of renal ischemia-reperfusion injury ,then re-moved the clamps 3.h ,6h ,12h ,24h after renal reperfusion ,the plasma and kidneys were collected for detec-ting the serum creatinine ,BUN level by auto biochemical analysis ,IL-32 ,TNF-αand IL-1βexpression in se-rum by ELISA assay ,activity of myeloperoxidase in kidney tissues by chemical chromatometry .Results :The levels of serum Cr and BUN in I/R group and IgG+I/R group were significantly higher than S group (P<0 0.5) ,but there is no significant difference between Anti-IL-32+I/R group and S group(P>0 0.5) .Similar-ly ,The levels of IL-32 ,TNF-αand IL-1βin I/R and IgG+I/R group were significantly higher than S group (P<0 0.5) ,but there is no significant difference between Anti-IL-32+ I/R group and S group(P>0 .05) . The activity of MPO reduced significantly (P<0 0.5) .Conclusions :IL-32 is highly expressed in serum and kidney on renal ischemia-reperfusion injury .Blockages of IL-32 could decrease the level of inflammatory ,in-hibit the movement of neutrophils ,and reduce the renal histopathologic damage .