Cloning of complete genes for novel hydrolytic enzymes from Antarctic sea water bacteria by use of an improved genome walking technique.

摘要

An improved gene identification technique and its application to genes encoding novel enzymes from Antarctic marine bacteria are reported. The technique uses PCR with Consensus-Degenerate Hybrid Oligonucleotide Primers (CODEHOP) and an improved genome walking method based on cassette-ligation. The developed technique was used to obtain complete sequences for 3 subtilases (P4, P6 and P7), a xylanase and a lipase.