Artificial spawning of carp Cyprinus carpio L.: differences between the effects on reproduction in females of Polish and Hungarian provenance treated with carp pituitary and (D-Ala~6) GnRH ProNHEt (Kobarelin)

摘要

Ovulation was stimulated in carp females of Polish and Hungarian provenance using injections of (D-Ala~6) GnRH ProNHEt (Kobarelin) (20 #mu#g kg~(-1)) with metoclopramide (10 mg kg~(-1)) or carp pituitary (3 mg kg~(-1)). For estimating the effects ofthe substance stimulating ovulation and of the female provenance, analysis of variance was carried out using the least squares method. It was found that the stimulating substance, provenance and interaction of these factors did not significantly affectthe weight of eggs obtained. On the other hand, the stimulating substance, provenance and interaction had a highly significant (P < = 0.0001) effect on the percentages of fertilization and live embryos. Females of Hungarian provenance treated with Kobarelin yielded eggs of much better quality but a smaller weight than after the pituitary treatment. In the case of carp females of Polish provenance, a yield of eggs of smaller weight and very poor quality was noted compared with the weight and quality of eggs obtained after hypophysation.