Method for selective detection and quantification of palitoxinas by their toxic effects on excitable cells.

摘要

Method for selective detection and quantification of palitoxinas by its toxic effect on excitable cells. The present invention relates to a method for the detection and quantification of systematic palytoxin and its derivatives (PLTXs) in test samples by utilizing excitable cells and a selective inhibitor of cytotoxicity PLTXs. Adding to the cells of test samples contaminated with PLTXs decreases cell viability in proportion to the concentration of existing PLTXs in the sample. The presence of inhibitor toxicity PLTXs during exposure of the cells to test samples contaminated with PLTXs specifically prevents toxicity. Comparison of survival levels of cells treated with the contaminated sample PLTXs respect to the values ​​obtained in cultures treated with varying concentrations of purified PLTX to determine the concentration present in lamuestra PLTXs problem.