An osmotic stress-inducible kinase functions as a negative regulator of osmotic stress signaling in plants

摘要

PURPOSE: Provided is phosphorylase AtSIK which inhibits an osmotic stress inducing gene in order to further increase the productivity of plants with greater resistance to the stress. CONSTITUTION: Phosphorylase AtSIK is obtained by the next steps of: i) cultivating Arabidopsis thaliana on MS plate at 22 deg.C in culture room or in greenhouse where the sample is placed under bright condition for 16 hours and under dark condition for 8-hour, repeatedly, with 70 % moisture; ii) manufacturing a subtraction library by comparing cDNA extracted from plant induced by salt stress with control cDNA and eliminating cDNA being always expressed; iii) collecting cDNA from the library randomly for base sequencing and obtaining 4 cDNA segments; and iv) selecting cDNA clones whose expression is controlled by osmotic stress and, selecting again a 2090bp cDNA which has an open reading frame encoding 557 amino acids, among the clones.